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对虎杖中蒽醌化合物进行了分级分离，并且对乙酸乙酯卒取部分晶Ⅲ、晶Ⅳ抗ＨＳＶ－１药效，通过病毒所致细胞病变的抑制以及空斑减数实验进行了观察。结果表明，在ＨＥＰ－２细胞系统中，晶Ⅳ对ＨＳＶ－１Ｆ株的增殖及其感染均具有明显的抑制作用，ＥＤ５０分别为０．０６７ｍｇ／Ｌ及０．０３０７２ｍｇ／Ｌ，ＥＤ９０分别为３．３９８ｍｇ／Ｌ及１．８１１ｍｇ／Ｌ。同样，晶Ⅳ对ＨＳＶ－１Ｆ株也具有显著的直接杀灭作用，ＥＤ５０及ＥＤ９０分别为０．０９４４３ｍｇ／Ｌ及３．５０１ｍｇ／Ｌ。认为晶Ⅳ对ＨＳＶ－１具有显著的抑制作用，而其细胞毒作用不强，值得开发利用。
The pupal compounds in Polygonum cuspidatum were fractionated, and the efficacy of partial crystal III and crystal IV anti-HSV-1 on ethyl acetate was observed, and the cytopathogenic inhibition by the virus and plaque reduction experiments were observed. The results showed that in the HEP-2 cell system, crystal IV had a significant inhibitory effect on the proliferation and infection of HSV-1F strains, with ED50 of 0.067 mg/L and 0.03072 mg/L, respectively, and ED90 was 3. 398 mg/L and 1.811 mg/L. Similarly, crystal IV also has a significant direct killing effect on the HSV-1F strain, with ED50 and ED90 of 0.09443 mg/L and 3.501 mg/L, respectively. It is considered that crystal IV has a significant inhibitory effect on HSV-1, but its cytotoxicity is not strong and it is worth exploiting.