With the AMMI (additive main effects and multiplicative interaction) analysis model, thedetermination of the sensitivity to temperature among different TGMS (thermo-sensitivegenic male sterile) lines was performed. To assess the genetic differences due to hightemperature stress at the fertility-sensitive stage (10-20d before heading), sevengenotypes (six TGMS lines and the control Pei-Ai64S) were grown from May 4 at sevendifferent stages with 10d intervals. The temperatures at the fertility-sensitive stagesinvolved twelve levels from<20 to>℃ under the regime natural conditions in Hangzhou,China. There was considerable variation in pollen fertility among genotypes in responseto high temperature. Five genotypes identified as TGMS lines as their percentages offertile pollens were lower than or close to that of the control except for the unstableline RTS19 (V6). When the temperatures at the fertility-sensitive stage were at Ⅰ-Ⅳ,Ⅴ-Ⅵ and Ⅶ-Ⅻ, the percentages of fertile pollens varied in the ranges of 46.46-48.49%,19.62-22.79% and 3.49-5.87%, respectively. The critical temperatures of sterility andfertility in the five TGMS lines were 25.1 and 23.0℃, respectively. Considering theamounts and directions of main effect and their IPCA (interaction principal componentsanalysis), we can classify the lines and temperature levels into different groups, anddescribe the characteristics of genotypetemperature interaction, offering the informationand tools for the development and utility of thermo-sensitive male sterile lines.Several TGMS rice lines with their reproductive sensitivity to high temperature that canbe screened using the AMMI model may add valuable germplasm to the breeding program ofhybrid rice. With the AMMI (additive main effects and multiplicative interactions) analysis model, the determination of the sensitivity to temperature among different TGMS (thermo-sensitivegenic male sterile) lines was performed. To assess the genetic differences due to hightemperature stress at the fertility-sensitive stage ( 10-20d before heading), seven genotypes (six TGMS lines and the control Pei-Ai64S) were grown from May 4 at sevendifferent stages with 10d intervals. The temperatures at the fertility-sensitive stages in about twelve levels from <20 to> ° C under the regime natural conditions in Hangzhou, China. There was considerable variation in pollen fertility among genotypes in response to high temperature. Five genotypes identified as TGMS lines as their percentages offertile pollens were lower than or close to that of the control except for the unstableline RTS19 (V6) . When the temperatures at the fertility-sensitive stage were at Ⅰ-Ⅳ, Ⅴ-Ⅵ and Ⅶ-Ⅻ, the percentages of fertile pollens varied in the ranges of 46.46-48.49%, 19.62-22.79% and 3.49-5.87%, respectively. respectively. The critical temperatures of sterility and fertility in the five TGMS lines were 25.1 and 23.0 ° C, respectively. Considering the amounts and directions of main effect and their IPCA (interaction principal componentsanalysis), we can classify the lines and temperature levels into different groups, anddescribe the characteristics of genotypetemperature interaction, offering the information and tools for the development and utility of thermo-sensitive male sterile lines. Sewer TGMS rice lines with their reproductive sensitivity to high temperature that canbe screened using the AMMI model may add valuable germplasm to the breeding program ofhybrid rice.