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目的:建立测定人血浆中多潘立酮浓度的高效液相色谱法。方法:以普萘洛尔为内标,取血浆样品用0.1 mol·L~(-1)氢氧化钠溶液碱化后乙醚提取,再用0.2 mol·L~(-1)盐酸溶液提取乙醚中药物,取20μl进行HPLC测定。色谱柱:Diamonsil C_(18)(200 mm×4.6 mm,5μm),流动相:乙腈-0.02 mol·L~(-1)磷酸盐缓冲液(pH 3.5)(25:75),荧光检测器激发波长:282nm;发射波长:326nm,流速:1.0 ml·min~(-1),柱温:25℃。结果:标准曲线的线性范围为1.0～100.0μg·L~(-1)(r=0.999 2),相对回收率在99.1%～108.8%之间,日内和日间RSD均小于10%。结论:该方法具有操作简便可靠,准确,稳定性高等特点,适用于多潘立酮血药浓度的测定。
Objective: To establish a HPLC method for the determination of domperidone in human plasma. Methods: Propranolol was used as an internal standard. The plasma samples were extracted with 0.1 mol·L -1 sodium hydroxide solution and extracted with diethyl ether and extracted with 0.2 mol·L -1 hydrochloric acid Drug, take 20μl for HPLC determination. The chromatographic conditions were as follows: Diamonsil C_ (18) (200 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile-0.02 mol·L -1 phosphate buffer (pH 3.5) Wavelength: 282nm; emission wavelength: 326nm, flow rate: 1.0 ml · min -1, column temperature: 25 ℃. Results: The linear range of the standard curve was 1.0-100.0 μg · L -1 (r = 0.999 2). The relative recoveries ranged from 99.1% to 108.8%. The RSDs were less than 10% within and between days. Conclusion: The method is simple, reliable, accurate and has high stability. It is suitable for the determination of domperidone plasma concentration.