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目的研究肠道病毒71型(EV71)3CD蛋白在致病机制中的作用。方法实验分为对照组、SDLY1组、SDLY107组和SDLY107(1-3CD)组。通过反向遗传技术将弱毒株SDLY1株的3CD蛋白编码区置换到强毒株SDLY107株,形成拯救病毒SDLY107(1-3CD)株。将EV71病毒接种于RD细胞和Vero细胞,于37℃和39.5℃孵育一定时间后,采用MTT试验检测活细胞量,以判断病毒对细胞损伤作用的改变。结果 37℃下,拯救病毒SDLY107(1-3CD)对细胞的损伤作用弱于SDLY107株,但仍强于SDLY1株(P<0.05);39.5℃下,拯救病毒SDLY107(1-3CD)株对细胞的损伤作用较SDLY107株下降(P<0.05),但与SDLY1株的差异无统计学意义(P>0.05)。结论 EV71 3CD编码区的置换可以改变病毒对细胞的损伤作用,3CD蛋白编码区可能存在影响病毒毒力的位点。这一发现有助于理解EV71的致病机制。
Objective To study the role of enterovirus 71 (EV71) 3CD in pathogenic mechanism. Methods The experiment was divided into control group, SDLY1 group, SDLY107 group and SDLY107 (1-3CD) group. The 3CD protein coding region of the attenuated strain SDLY1 was replaced by the virulent strain SDLY107 by reverse genetic technology to form the rescue virus SDLY107 (1-3CD) strain. The EV71 virus was inoculated into RD cells and Vero cells, and after incubation at 37 ° C and 39.5 ° C for a certain period of time, the amount of viable cells was measured by MTT assay to determine the effect of virus on cell injury. Results At 37 ℃, the rescue effect of SDLY107 (1-3CD) on cells was weaker than that of SDLY107, but was still stronger than that of SDLY1 (P <0.05). At 39.5 ℃, SDLY107 (1-3CD) (P <0.05), but no significant difference with SDLY1 strain (P> 0.05). Conclusion The substitution of EV71 3CD coding region can change the damage effect of virus on cells. The coding region of 3CD protein may affect the virulence of virus. This finding helps to understand the pathogenesis of EV71.