论文部分内容阅读
血清中的微量锌和铜是生物化学和临床医学研究的重要元素。近年的研究发现,人类血清中铜锌含量有一定比值,此比值与人体代谢和病理变化密切相关,因而测定血清的铜锌比值比单独测定血清中铜或锌的含量更有实用价值。本文报告在pH 8.5～9.0缓冲溶液中,使Zn~(2+)、Cu~(2+)与PAN生成红色不溶性络合物,选乳化剂OP增溶,再利用两络合物对二乙基氨基二硫代甲酸钠(DDTC)的稳定性不同而连续测定血清中锌和铜。与报导的测定
Trace amounts of zinc and copper in serum are important elements in biochemical and clinical medicine research. Recent studies have found that there is a certain ratio of copper and zinc in human serum, which is closely related to human metabolism and pathological changes. Therefore, it is more practical to measure the ratio of copper to zinc in serum than to measure the content of copper or zinc in serum alone. In this paper, we report the red insoluble complex of Zn 2+, Cu 2+ and PAN in pH 8.5-9.0 buffer solution and the solubilization of OP, Serum zinc and copper are continuously measured as a function of the stability of sodium dithiocarbamate (DDTC). With the reported assay