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目的:通过接种W256癌肉瘤建立Wistar大鼠肝癌转移模型,观察康莱特注射液与肝动脉结扎法对大鼠荷瘤脾重与肝癌转移灶的抑制效果,并测定其对瘤组织内基质金属蛋白酶(MMP-1)及肝细胞生长因子(HGF)表达的影响,探讨中西医结合防治肝癌转移的新途径。方法:在Wistar大鼠脾下极注射W256癌肉瘤细胞悬液,建立肝癌转移模型。随机分为4组:对照组、动脉结扎组、康莱特组、康莱特+动脉结扎组。计算各组瘤重抑制率与肝癌转移抑制率,采用原位杂交技术检测肿瘤细胞内MMP-1和HGFmRNA表达水平。结果:康莱特和康莱特加动脉结扎组均显著抑制脾脏肿瘤生长并抑制肝脏瘤组织MMP-1mRNA表达,康莱特组能明显抑制肝癌转移灶产生,各治疗组大鼠瘤细胞HGFmRNA表达水平均低于对照组。结论:康莱特注射液可显著抑制原发灶生长与肝癌转移灶形成,抑制瘤细胞MMP-1表达是其抗肝转移的重要机制;康莱特注射液结合动脉结扎可使瘤细胞MMP-1表达减少,提示应优化两者结合途径。
OBJECTIVE: To establish Wistar rat hepatocarcinoma metastasis model by inoculating W256 carcinosarcoma, and to observe the inhibitory effect of KLT injection and hepatic artery ligation on tumor-bearing spleen mass and metastasis of hepatocellular carcinoma in rats, and to determine its effect on the expression of matrix metalloproteinase (MMP-1) and hepatocyte growth factor (HGF) expression, to explore the combination of traditional Chinese and Western medicine prevention and treatment of liver cancer metastasis of new ways. Methods: The W256 carcinosarcoma cell suspension was injected into the spleen of Wistar rats to establish a model of liver cancer metastasis. Randomly divided into 4 groups: control group, artery ligation group, Kanglaite group, Kanglaite + artery ligation group. The inhibition rate of tumor weight and the rate of liver cancer metastasis were calculated. The expression of MMP-1 and HGF mRNA in tumor cells was detected by in situ hybridization. Results: Both KLT and KLT group significantly inhibited the growth of spleen and inhibited the expression of MMP-1 mRNA in hepatic tumor tissues. The KLT group could significantly inhibit the metastasis of hepatocellular carcinoma. The expression levels of HGF mRNA in tumor cells in each treatment group were significantly lower In the control group. CONCLUSION: Kanglaite injection can significantly inhibit the growth of primary tumor and metastasis of hepatocellular carcinoma, and inhibit the expression of MMP-1 in tumor cells as an important mechanism of its anti-hepatic metastasis. Kanglaite injection combined with arterial ligation can make the expression of MMP-1 Reduce, suggesting that the combination of the two should be optimized.