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目的 探讨细胞色素b2 4 5(P2 2 phox)C2 4 2T基因突变频率及该基因多态性与血浆中维生素E和冠心病的关系。方法 应用聚合酶链反应及限制性片断长度多态性 (PCR RFLP)方法检测P2 2 phox基因型在冠心病患者中频率的分布 ,应用反相高效液相色谱法 (RP HPLC)检测血浆中维生素E浓度。结果 在总的研究人群中P2 2 phox基因的C和T两种等位基因分布频率为 92 72 %和 7 2 8% ;C2 4 2T多态性与冠心病发生有关 ;血浆中高维生素E水平对冠心病的发生有保护作用 ,二者对冠心病的发生呈负相乘模型交互作用。Logistic分析结果提示 ,男性、吸烟、不饮酒、高血压病史、糖尿病史、父母中风史及血浆中低维生素E浓度为冠心病的独立危险因素。结论 P2 2 phoxC2 4 2T多态性与冠心病发生有关 ,血浆中高维生素E水平在冠心病的发生有保护作用 ,二者在冠心病的发生中具有交互作用
Objective To investigate the relationship between the mutation frequency of C2 2 2T2 gene and the polymorphism of vitamin B2 and coronary heart disease in plasma. Methods The frequency distribution of P2 2 phox genotypes in patients with coronary heart disease was detected by polymerase chain reaction and restriction fragment length polymorphism (PCR RFLP). RP-HPLC was used to detect plasma vitamin E concentration. Results The frequencies of C and T alleles of P2 2 phox gene were 92 72% and 728% in the overall study population. The C2 4 2T polymorphism was associated with CHD. The plasma level of high vitamin E The occurrence of coronary heart disease has a protective effect, the two have a negative multiplication model interaction of coronary heart disease. Logistic analysis suggested that men, smoking, non-alcoholism, history of hypertension, history of diabetes, history of parental stroke and plasma low-vitamin E concentrations were independent risk factors for coronary heart disease. Conclusion The P2 2 phoxC2 4 2T polymorphism is associated with the development of coronary heart disease. The plasma levels of high vitamin E may play a protective role in the pathogenesis of coronary heart disease. Both of them have an interaction in the pathogenesis of coronary heart disease