Background: Compared to the West countries, Chinese patients present a special primary disease spectrum, diverse valvular pathogenesis, and different postoperational anticoagulation strategy.This research aimed to evaluate the mid-to long-term clinical performance of Hancock Ⅱ bioprosthesis in the Chinese population.Methods: This study retrospectively reviewed all patients who received surgical treatments with at least one Hancock Ⅱ bioprosthesis implantation from January 2004 to December 2013 at a single center in China.Totally 647 patients were included in the clinical evaluation, and 629 patients were successfully discharge, among whom 605 patients were completely followed-up.The follow-up rate was 96.2％.The mean and median follow-up time was 62.0 ± 59.0 and 56.0 months, respectively Postoperative outcomes of survival rates, reoperations and valve related morbidities were assessed.Continuous and categorical variables were compared using the t-test and Chi-square test, respectively.Survival and freedom from adverse events were calculated by using a Kaplan-Meier method.Results: The overall in-hospital mortality was 2.8％ (18/647) while there were 34 deaths (5.6％, 34/605) in the follow-up stage after discharge.The overall survival rate was 94.6％ and 82.7％ at 5 years and 10 years, respectively The cumulative survival rate of 10 years was 82.8％ in AVR group, 84.4％ in MVR group, and 78.4％ in DVR group.The overall rate of freedom from reoperations was 95.5％ at 5 years and 86.8％ at 10 years.The freedom from reoperation at 10 years was 87.0％, 88.1％, and 84.0％ in AVR, MVR, and DVR group, respectively.The freedom from morbidities at 10 years was: 90.3％ for thromboembolism, 95.2％ for hemorrhage, 97.5％ for prosthesis endocarditis, 95.9％ for paravalvular leak, and 94.6％ for structural valve deterioration, respectively.Conclusions: Hancock Ⅱ bioprosthesis exhibited a satisfactory mid-to long-term durability and promising clinical performance in the Chinese population.The occurrence rates of death and other adverse events in this single-center study were overall coincident and quite acceptable when compared with existing data.