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目的：研究血小板生成素（ＴＰＯ）基因注射法对健康小鼠血小板的促生成作用。方法：将带有ｈＴＰＯｃＤ－ＮＡ的ｐｃＤＮＡ３质粒按６０μｇ／只剂量注射到１７５只昆明小鼠后肢内侧肌肉内，每天断尾采血计数白细胞和血小板，观察不同时间的骨髓和脾脏组织学变化。周期未注射小鼠做为对照组。结果：在基因注入后的３ｄ起即出现血小板和巨核细胞增多。在所观察的２７ｄ内，血小板有一可持续１周的高水平期，平均约为同期对照的１８４％，最高可达３倍以上。计数高峰后血小板在高于对照水平上呈波动状态。小鼠脾脏出现以巨细胞增生和内皮细胞活化为特点的组织学变化。结论：ＴＰＯ基因注射可刺激血小板和巨核细胞增殖，同时提示ＴＰＯ具有刺激免疫功能的作用。
Objective: To investigate the effect of thrombopoietin (TPO) gene injection on platelet production in healthy mice. Methods: The pcDNA3 plasmid with hTPOcD-NA was injected into the medial hind limbs of 175 Kunming mice at a dosage of 60 μg / L. The white blood cells and platelets were counted every day and the histological changes of bone marrow and spleen at different time points were observed. Cycle non-injected mice as a control group. Results: Platelets and megakaryocytes increased at 3 days after gene injection. During the 27 days observed, there was a high level of platelets that lasts for 1 week, averaging about 184% of the control for the same period, up to 3 times more. Platelets fluctuated above the control level after the peak count. Histological changes characterized by giant cell proliferation and endothelial cell activation occurred in the spleen of mice. Conclusion: TPO gene injection can stimulate platelet and megakaryocyte proliferation, suggesting that TPO can stimulate immune function.