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目的了解云南省红河州弥勒县蚊虫中虫媒病毒的分布特点,为虫媒病毒病防治提供科学依据。方法 2009年7月在弥勒县采集蚊虫标本,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果从采集到的三带喙库蚊4400只和中华按蚊2200只中分离到9株阳性分离物,经分子生物学鉴定,7株分离物为流行性乙型脑炎(乙脑)病毒(JEV),均分离自三带喙库蚊;2株分离物为淡色库蚊浓核病毒(CppDNV),分离自三带喙库蚊和中华按蚊。结论首次在红河州弥勒县分离到乙脑病毒,从病原学证实弥勒县是乙脑流行区。流行株同源性和分子遗传进化分析证实为基因Ⅰ型乙脑病毒,为滇东南地区首次分离。
Objective To understand the distribution characteristics of arboviruses in mosquitoes in Maitreya County, Honghe Prefecture, Yunnan Province, and to provide a scientific basis for the prevention and treatment of arboviral viruses. Methods In July 2009, mosquitoes were collected from Maitreya County. The virus was isolated by cell culture and the virus isolates were identified by molecular biology. Results A total of 9 positive isolates were isolated from 4,400 Culex tritaeniorhynchus and 2200 Anopheles sinensis collected. The positive isolates were identified by molecular biology. Seven isolates were identified as JE virus JEV) were isolated from Culex tritaeniorhynchus; two isolates were CpDNV, isolated from Culex tritaeniorhynchus and Anopheles sinensis. Conclusions JE virus was isolated in Mile County, Honghe Prefecture for the first time. Etiology confirmed that Mile County is endemic to JE. Epidemiology and molecular genetic evolution analysis of epidemic strains confirmed as genotype I JE virus, which was the first isolate in Southeast Yunnan.