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一、培养基准备1.培养基制作将马铃薯洗净去皮,切成5毫米方块。称取200克放入铝锅中,加水1000毫升后煮沸20～30分钟,用4层纱布过滤取滤液,将滤液倒入锅中再加入琼脂20克,用小火加热不断搅拌,至琼脂完全融化后,加入葡萄糖20克和蛋白胨10克、磷酸二氢钾2克、硫酸镁0.7克,搅拌至完全融化,倒入量筒补水至1000毫升。2.装试管将制作好的培养基,趁热用漏斗分装试管,装入试管长度的1/4。培养基液体不得沾附管口壁。塞上硅胶塞,将试管每7支用棉线扎成把。
First, the medium preparation 1. Medium production will be washed and peeled potatoes, cut into 5 mm square. Weigh 200 grams into the aluminum pan, add 1000 ml of water and boil 20 to 30 minutes, with four layers of gauze filter to take the filtrate, the filtrate into the pot and then add 20 grams of agar, with small heat stirring constantly Agar After thawing, 20 g of glucose and 10 g of peptone, 2 g of potassium dihydrogen phosphate and 0.7 g of magnesium sulfate were added and the mixture was stirred until it completely melted. The mixture was poured into a graduated cylinder and rehydrated to 1000 ml. 2. Test tube will be installed to make a good medium, hot tube dispensing funnel, into the tube length of 1/4. Media Liquid must not adhere to the orifice wall. Plug the silicone plug, the test tube every 7 with a cotton tie into the handle.