论文部分内容阅读
目的观察镍与铬对小鼠睾丸的联合毒作用,探讨镍、铬联合作用类型及毒作用机制。方法54只昆明种雄性小鼠按3×3析因设计随机等分为9组,镍染毒剂量为0.0,2.5、5.0mg/kg·bw、铬染毒剂量为0.0、2.5、5.0 mg/kg·bw,按0.1 ml/10 g·bw连续灌胃5d,35d后处死动物,检测睾丸脏器系数、睾丸结构、睾丸细胞凋亡及细胞周期;精子数量、活力和畸型;检测睾丸组织中MDA含量、SOD、LDH、SDH活性。结果小鼠睾丸脏器系数、SOD、LDH、SDH活性随染毒剂量增加而降低,MDA含量增高(P<0.01),且有量一效关系;精子数量较对照组低(P<0.01)、精子活力下降(P<0.01)、畸形率高于对照(P<0.01),睾丸的结构受损,各染毒组小鼠睾丸细胞凋亡率和G_0/G_1期细胞百分数较对照组升高(P<0.05),各染毒组s期及G_2+M期细胞百分数较对照组低(P<0.05),并呈现一定的量一效关系,镍、铬合用的效应大于二者单独作用的效应之和(P<0.01)。结论镍和铬联合染毒可致小鼠睾丸损伤,二者的联合作用为协同毒效应;损伤机制可能与其降低睾丸细胞的抗氧化能力、抑制睾丸细胞酶活性、促进睾丸细胞凋亡等有关。
Objective To observe the combined toxicity of nickel and chromium on the testis of mice and to explore the combined action types of nickel and chromium and their toxic mechanism. Methods Fifty-four male Kunming mice were randomly divided into 9 groups according to 3 × 3 factorial design. The doses of nickel were 0.0, 2.5 and 5.0 mg / kg · bw respectively, and the doses of chromium were 0.0, 2.5 and 5.0 mg / kg · bw, 0.1g / 10g · bw continuous gavage 5d, 35d after the animals were sacrificed, testis organ coefficient, testicular structure, testicular cell apoptosis and apoptosis; sperm count, vitality and abnormalities; testicular tissue MDA content, SOD, LDH, SDH activity. Results The activity of SOD, LDH and SDH in the testis decreased with the increase of the dose and the content of MDA in the testis (P <0.01), and had a dose-effect relationship. The sperm count was lower than that in the control group (P <0.01) Sperm motility decreased (P <0.01), deformity rate was higher than that of the control (P <0.01), and the structure of testis was impaired. The percentage of testicular cell apoptosis and G 0 / G 1 phase increased (P <0.05). The percentage of cells in s phase and G 2 + M phase in each treatment group was lower than that in control group (P <0.05), and showed a certain amount-effect relationship. The combined effect of nickel and chromium was greater than the effect of the two alone (P <0.01). Conclusions The combination of nickel and chromium can induce the testicular damage in mice. The combined effect of them is synergistic toxic effect. The mechanism may be related to reducing the antioxidant capacity, inhibiting the activity of testicular cells and promoting the apoptosis of testicular cells.