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本实验观察了海群生在体外对彭亨丝虫第三期幼虫的作用。采自感染14天的埃及伊蚊体内的彭亨丝虫第三期幼虫,以80～100条为一组接种于盛有10ml RPMI 1640培养液(内加10％灭活胎牛血清与恒河猴肾细胞)的培养瓶内,置37℃培养。接种后每隔一天更换一次培养液,每6天转种一次,至第22天终止培养。存活幼虫经固定后测定大小,并区分发育阶段。结果表明,培养至22天,未加海群生的对照组中幼虫存活率为51.1％～74.2％,其中77.8％的幼虫发育至第四期,幼虫的平均长
In this study, we observed the effects of seaquats on the third larva of Penaeid Wilt in vitro. The third larva of Paenias bacterioides collected from Aedes aegypti for 14 days was inoculated in a volume of 80-100 in 10 ml RPMI 1640 medium supplemented with 10% inactivated fetal bovine serum and rhesus monkey kidney Cells) flask, set at 37 ℃ culture. Every other day after inoculation, the medium was changed, transplanted every 6 days and stopped on the 22nd day. Survival larvae were sized and sized to differentiate between developmental stages. The results showed that the survival rate of larvae in the control group without sea group was 51.1% ~ 74.2% after 22 days of cultivation, of which 77.8% larvae developed to the fourth larvae