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目的:探讨人参皂苷单体Rh2对人鼻咽癌CNE-2S细胞增殖及凋亡的影响。方法:将生长在对数期的人鼻咽癌CNE-2S细胞分为空白对照组、阴性对照组和实验组。对照组常规培养,阴性对照组采用含有DMSO的培养液培养,实验组在对照组细胞的基础上加入不同浓度人参皂苷单体Rh2处理。采用MTT法测定细胞增殖,PI单染流式细胞术分析各时期细胞所占百分比,Annexin V-PI双染流式细胞仪检测细胞的凋亡情况。结果:与阴性对照组相比,实验组各浓度下的Rh2对CNE-2S细胞均具有显著的增殖抑制作用(P<0.05),且随着Rh2浓度的增加而呈现增强的趋势,其中浓度为12.5 mg·L-1 Rh2增值抑制率最低,浓度为100 mg·L-1Rh2增值抑制率最高。不同浓度人参皂苷单体Rh2 G0/G1期细胞分布显著高于阴性对照组(P<0.001),且G2/M、S期细胞比例显著低于阴性对照组(P<0.01),且随着人参皂苷单体Rh2浓度的增加作用呈现增强的趋势(P<0.05);不同浓度的Rh2单体作用24h,CNE-2S细胞早期、晚期凋亡率及总凋亡率均较阴性对照组明显增高(P<0.001),并且在Rh2单体浓度为100 mg·L-1时,凋亡率最高。结论:人参皂苷单体Rh2对人鼻咽癌CNE-2S细胞增殖及凋亡具有显著的影响,并且可能对单体Rh2的浓度存在依懒性。
Objective: To investigate the effects of ginsenoside Rh2 on the proliferation and apoptosis of human nasopharyngeal carcinoma CNE-2S cells. Methods: Human nasopharyngeal carcinoma CNE-2S cells growing in logarithmic phase were divided into blank control group, negative control group and experimental group. The control group was cultured routinely. The negative control group was cultured in medium containing DMSO. The experimental group was treated with different concentrations of ginsenoside monomer Rh2 on the basis of the control group cells. The cell proliferation was determined by MTT assay. The percentage of cells in each stage was analyzed by PI single-flow cytometry. The apoptosis of cells was detected by Annexin V-PI double staining flow cytometry. Results: Compared with the negative control group, Rh2 had a significant inhibitory effect on CNE-2S cells (P <0.05), and showed an increasing trend with the increase of Rh2 concentration 12.5 mg · L-1 Rh2 had the lowest value of proliferation inhibition, the highest concentration of 100 mg · L-1Rh2 had the highest value-added inhibition. The cell distribution of ginsenoside monomer Rh2 G0 / G1 phase at different concentrations was significantly higher than that of negative control group (P <0.001), and the proportion of cells in G2 / M and S phase was significantly lower than that of negative control group (P <0.01) (P <0.05). Rh2 concentration of different concentrations of Rh2 monomer for 24h, early and late apoptosis rate and CNE-2S cell apoptosis rate was significantly higher than the negative control group (P <0.05) P <0.001), and the highest apoptosis rate was found when Rh2 monomer concentration was 100 mg · L-1. CONCLUSION: Ginsenoside Rh2 can significantly affect the proliferation and apoptosis of human nasopharyngeal carcinoma cell line CNE-2S, and may be due to the laziness of monomer Rh2 concentration.