【摘 要】
：
KangFuXinYe (KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China.However,its therape
【机 构】
：
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Metab
论文部分内容阅读
KangFuXinYe (KFX),the ethanol extract of the dried whole body of Periplaneta americana,is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China.However,its therapeutic effect and mechanism are not yet well understood.Thus,the aim of this study was to investigate the gastroprotective effects ofKangFuXinYe (KFX) in indomethacin-induced gastric damage.Rats were randomly divided into six groups as follows:control,treated with indomethacin (35 mg.kg-1),different dosages of KFX (2.57,5.14 and 10.28 mL.kg-1,respectively) plus indomethacin,and sucralfate (1.71 mL·kg-1) plus indomethacin.After treatment,rat serum,stomach and gastric homogenates were collected for biochemical tests and examination of histopathology firstly.Rat serum was further used for metabolomics analysis to research possible mechanisms.Our results showed that KFX treatment alleviated indomethacin-induced histopathologic damage in rat gastric mucosa.Meanwhile,its treatment significantly increased cyclooxygenase-1 (COX-1),prostaglandin E2 (PGE2) and epidermal growth factor (EGF) levels in rat serum and gastric mucosa.Moreover,KFX decreased cyclooxygenase-2 (COX-2) and intedeukin-6 (IL-6) levels.Nine metabolites were identified which intensities significantly changed in gastric damage rats,including 5-hydroxyindoleacetic acid,indoxylsulfuric acid,indolelactic acid,4-hydroxyindole,pantothenic acid,isobutyryl carnitine,3-methyl-2-oxovaleric acid,sphingosine l-phosphate,and indometacin.These metabolic deviations came to closer to normal levels after KFX intervention.The results indicate that KFX (10.28 mL.kg-1) exerts protective effects on indomethacin-induced gastric damage by possible mechanisms of action (regulating tryptophan metabolism,protecting the mitochondria,and adjusting lipid metabolism,and reducing excessive indomethacin).
其他文献
Glycyrrhetinic acid (GA) is the bioactive ingredient in Giycyrrhizae Radix et Rhizoma.Our previous study has reported that GA has protective effect on realgar-induced bepatotoxicity.However,the details of the hepatoprotective mechanisms of GA on realgar-i
Three new isocoumarin derivatives,(S)-6,8-dihydroxy-5-(methoxymethyl)-3,7-dimethylisochroman-1-one (1),(S)-6,8-dihydroxy-3,5,7-trimethyl-isochroman-l-one (2) and (R)-2-chloro-3-(8-hydroxy-6-methoxy-1-oxo-1H-isochromen-3-yl)propyl acetate (3),along with fo
Two new meroterpenoid compounds (1 and 2) together with five known meroterpenoid derivatives (3-7) were isolated from solid culture of mushroom Panus lecomtei.The structures of new compounds were confirmed by the analysis of NMR and HR-ESI-MS spectroscopi
In the atmosphere of the earth,many gases including nitrogen (N2),oxygen (O2),and carbon dioxide (CO2) naturally exist to support universal lives.Interestingly,in mammals and plants,some gases are produced endogenously and are known to play essential role
Glaucoma is a disease that causes irreversible blindness.Reducing intraocular pressure (IOP) is the main treatment at present.Nitric oxide (NO),an endogenous gas signaling molecule,can increase aqueous humor outflow facility,inhibit aqueous humor producti
Two new caryophyllene-type sesquiterpenoids,pestathenols A (1) and B (2) and one new α-furanone,pestatheranone A (6),along with five known compounds (3-5,7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis thea
The objective of this study was to verify the protective effect ofBifidobacterium longum (BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet (HFD) induced hepatic injury in mice.We also want to explore the mechanism of Selen
Hydrogen sulfide (H2S) is commonly referred to as the third gasotransmitter with firmly established physiological roles.Prodrug approaches have been broadly applied to deliver H2S for various applications and mechanistic studies.Since S-persulfidation and
Purpurolides D-F (1-3),three new polyoxygenated bergamotanes bearing a 6/4/5/5 tetracyclic ring system,were isolated from the endophytic fungus Penicillium purpurogenum IMM 003.Their structures were unambiguously elucidated based on extensive spectroscopi
Diterpenoid lactones (DLs),a group of furan-containing compounds found in Dioscorea bulbifera L.(DB),have been reported to be associated with hepatotoxicity.Different hepatotoxicities of these DLs have been observed in vitro,but reasonable explanations fo