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目的对金疮小草的化学成分进行研究。方法采用硅胶吸附柱色谱、ODS柱色谱、制备薄层色谱和半制备型高效液相色谱分离纯化,根据1H-NMR、13C-NMR等谱学数据进行结构鉴定。结果从金疮小草干燥全草的甲醇提取物的乙酸乙酯萃取物中分离得到15个单体化合物,分别鉴定为ajugacumbin A(1)、ajugacumbin B(2)、ajuganipponin B(3)、ajugamarin F4(4)、ajugarin I(5)、ajugamarin A1(6)、ajugamarin A1 chlorhydrin(7)、芹菜素(apigenin,8)、木犀草素(luteolin,9)、金合欢素(acacetin,10)、咖啡酸甲酯(methyl caffeate,11)、4-hydroxy-4-(3-oxo-1-butenyl)-3,5,5-trimethylcyclohex-2-en-1-one(12)、香草酸(vanillic acid,13)、黑麦草内酯(loliolide,14)和(10E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid(15)。结论其中化合物11和12为首次从筋骨草属中分离得到,化合物7和15为首次从金疮小草中分离得到。
Objective To study the chemical constituents of Scutellaria barbata. Methods The compounds were isolated and purified by silica gel column chromatography, ODS column chromatography, preparative thin layer chromatography and semi-preparative high performance liquid chromatography (HPLC). The structures were confirmed by 1H-NMR and 13C-NMR. Results Fifteen monomer compounds were isolated from the ethyl acetate extract of the methanolic extract of A. purpurea and identified as ajugacumbin A (1), ajugacumbin B (2), ajuganipponin B (3), ajugamarin F4 Ajugarin I (5), ajugamarin A1 (6), ajugamarin A1 chlorhydrin (7), apigenin 8, luteolin 9, acacetin 10, Methyl caffeate (11), 4-hydroxy-4- (3-oxo-1-butenyl) -3,5,5-trimethylcyclohex- 2-en-1-one (12), vanillic acid , 13), loliolide (14) and (10E, 15Z) -9,12,13-trihydroxyoctadeca-10,15-dienoic acid (15). Conclusion Compounds 11 and 12 were isolated from the genus Gastrodia for the first time, and compounds 7 and 15 were isolated from the genus Scutellaria for the first time.