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目的检测P-AKT(Ser473)蛋白、HER-2、P53在人乳腺良、恶性病变组织中的表达,分析P-AKT、HER-2、P53在乳腺肿瘤进展中的作用及临床意义,探讨P-AKT蛋白、HER-2、P53之间的关系。方法应用免疫组化Envision法,检测P-AKT蛋白、HER-2、P53在10例乳腺增生症、19例乳腺非典型增生疾病、13例乳腺导管原位癌及26例乳腺浸润性导管癌(IDC)中的表达,结果用SPSS12.0软件分析。结果 P-AKT蛋白、HER-2、P53在乳腺浸润性导管癌的表达(57.6%、65.3%、57.6%)和原位癌的表达(46.2%、61.6%、53.8%)显著高于在乳腺非典型增生性疾病(26.3%、5.3%、0.0%)及乳腺良性增生症的表达(0.0%,P<0.05)。P-AKT蛋白、HER-2和P53在乳腺浸润性导管癌的表达与其在乳腺原位癌中的表达之间表达差异无统计学意义(P>0.05);三者在乳腺增生症与非典型增生之间的表达差异均无统计学意义(P>0.05);三者在有无淋巴结转移的浸润性乳腺导管癌之间的表达差异无统计学意义(P>0.05)。在乳腺良、恶性病变中P-AKT蛋白的表达与HER-2、P53的表达均有相关性(P<0.01)。结论 P-AKT(Ser473)蛋白、HER-2、P53的高表达在乳腺癌发生、发展起重要作用,并且之间存在显著的正相关性。
Objective To detect the expression of P-AKT (Ser473), HER-2 and P53 in benign and malignant human breast tissues and to analyze the role and clinical significance of P-AKT, HER-2 and P53 in the progression of breast tumors. -AKT protein, HER-2, P53. Methods Immunohistochemical Envision method was used to detect the expression of P-AKT protein, HER-2 and P53 in 10 cases of hyperplasia of breast, 19 cases of atypical hyperplasia of breast, 13 cases of ductal carcinoma in situ and 26 cases of infiltrating ductal carcinoma of breast IDC), the results were analyzed by SPSS12.0 software. Results The expressions of P-AKT protein, HER-2 and P53 were significantly higher in breast invasive ductal carcinoma (57.6%, 65.3%, 57.6%) and in situ carcinoma (46.2%, 61.6%, 53.8% Atypical proliferative diseases (26.3%, 5.3%, 0.0%) and benign breast hyperplasia (0.0%, P <0.05). The expression of P-AKT protein, HER-2 and P53 in breast invasive ductal carcinoma and their expression in breast cancer in situ was not statistically different (P> 0.05); The three in breast hyperplasia and atypical There was no significant difference between the three groups (P> 0.05). There was no significant difference in the expression of these three between invasive ductal carcinoma with or without lymph node metastasis (P> 0.05). The expression of P-AKT protein in benign and malignant breast lesions correlated with the expression of HER-2 and P53 (P <0.01). Conclusion The overexpression of P-AKT (Ser473), HER-2 and P53 plays an important role in the occurrence and development of breast cancer, and there is a significant positive correlation between them.