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采用免疫组织化学方法．观察脐血细胞体外培养与扩增前后ＣＤ３４~＋细胞的比例以及ＨＬＡ抗原的表达。结果表明：脐血ＣＤ３４~＋细胞的百分比以及ＨＬＡ抗原的表达于体外培养前后无显著性变化（Ｐ＞０．０５），而在细胞因子的联合作用下，经一定时间的扩增后，ＣＤ３４~＋细胞百分比下降（Ｐ＜０．０１），ＨＬＡ抗原的表达有一定程度的提高。提示：体外扩增对脐血ＣＤ３４~＋细胞的比例以及ＨＬＡ抗原的表达有一定的影响，而未加细胞因子的体外直接培养则不产生明显影响。
Using immunohistochemistry. The ratio of CD34 + cells and the expression of HLA antigens in cord blood cells before and after culture and expansion were observed. The results showed that the percentage of CD34 + cells and the expression of HLA antigens in cord blood had no significant change before and after culture in vitro (P> 0.05). However, under the combined action of cytokines, CD34 ~ + Cells decreased (P <0.01), the expression of HLA antigen increased to a certain extent. Hint: In vitro expansion of cord blood CD34 ~ + cell ratio and the expression of HLA antigen have a certain impact, but without cytokines in vitro direct culture did not have a significant effect.