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本文测定了糖尿病患者尿中α_1-m和β_2-m的浓度,以探讨它们在糖尿病早期诊断中的意义。 材料和方法 一、材料: (一)α_1-m试剂盒由中国原子能科学研究院同位素研究所提供;β_2-m由中国北方免疫试剂研究所提供。 (二)FMJ-87型γ计数器。 二/对象和方法:正常对照组34例(男性21例,女性13例),年龄34～46岁。糖尿病组53例(男性30例,女性23例),年龄49～61岁。所有受检者均排去晨尿后收集尿液,一小时后测定α_1-m和β_2-m都采用放射免疫分析法测定,所有操作均按操作说明书进行。 结果 糖尿病患者尿中β_2-m浓度与正常对照组相比,儿显著性差异(p<0.05)而α_1-m浓度与正常对照组
In this paper, the urinary α_1-m and β_2-m concentrations in diabetic patients were measured to explore their significance in the early diagnosis of diabetes. MATERIALS AND METHODS I. Materials: (a) The α_1-m kit was provided by the Institute of Isotopes of China Institute of Atomic Energy; β_2-m was provided by the Institute of Immunological Reagents, North China. (B) FMJ-87 type γ counter. Second, objects and methods: 34 cases of normal control group (21 males and 13 females), aged 34 to 46 years. Diabetic patients 53 cases (30 males and 23 females), aged 49 to 61 years old. All subjects were discharged to the urine after morning urine collection, an hour later measured α_1-m and β_2-m were determined by radioimmunoassay, all operations were carried out according to the operating manual. Results The urinary β_2-m concentration in diabetic patients was significantly lower than that in normal controls (p <0.05), while the concentration of α_1-m in urine of diabetic patients was significantly lower than that in normal controls