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雌核灭活是雄核发育的第一个关键步骤,为研究紫外线对斑马鱼卵的雌核灭活效果,将斑马鱼的卵采用干法或置于纯水、Ringer氏溶液和Cortland鱼用生理盐水进行紫外线照射,结果表明,各种方法中均得到畸形鱼苗,并经染色体检查大多为单倍体;四种方法中在Cortland鱼用生理盐水中灭活效果最好,120 mJ·cm-2紫外线可以使雌核完全灭活;干法处理不能达到雌核完全灭活的目的,在纯水中灭活后的卵子受精率较低.而延迟受精的结果也表明,盐度较高的Cortland鱼用生理盐水可以较好的保护卵子,维持较长时间的受精能力,和其它处理方法有明显的差异.
Inactivation of gynogenes is the first key step in the development of the nucleus. In order to study the inactivation effect of ultraviolet rays on the zygotes of zebrafish eggs, the zebrafish eggs were dried or placed in pure water, Ringer’s solution and Cortland fish Physiological saline irradiation, the results showed that all kinds of methods are deformed fry, and most of the chromosome examination by haploid; four methods in the Cortland fish with the best inactivation of saline, 120 mJ · cm- 2 UV can make the gynogellis completely inactivated; dry treatment can not achieve the purpose of complete inactivation of the gynopia, and the inoculation rate of the egg inactivated in pure water is lower, while the result of delayed fertilization also shows that the salinity Cortland fish with saline can better protect the eggs, to maintain a longer time of fertilization, and other treatment methods were significantly different.