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目的观察三苯双脒(TBD)、青蒿琥酯(AS)、阿苯达唑(ABZ)、甲苯咪唑(MBZ)单用或联用治疗感染华支睾吸虫大鼠的疗效。方法 140只大鼠各感染华支睾吸虫囊蚴100个,于感染后第5周开始分组治疗,均采用灌胃给药。感染大鼠随机分为药物治疗组同批感染对照组(每组5～10只),将TBD、AS、ABZ与MBZ分别按照高、低剂量单剂用药和两两配伍给药,顿服,观察疗效。治疗后10d处死大鼠,解剖,收集胆管和肝组织内的华支睾吸虫,计算平均虫数和减虫率,对相应组间的平均虫数进行单因素方差分析。结果感染华支睾吸虫大鼠用75mg/kg体重TBD、MBZ、ABZ单剂治疗后,平均减虫率分别为98.04%、100%和86.67%,30mg/kg体重AS治疗后减虫率为87.25%;当给药剂量均降低1/3时,上述各药物治疗组的平均减虫率分别降至78.3%、46.02%、31.94%和62.07%。50mg/kg体重TBD分别与50mg/kg体重MBZ、ABZ配伍用组平均减虫率为98.43%和100%,检虫数与75mg/kg体重TBD、MBZ、ABZ单用药组比较,差异有统计学意义(P<0.05)。20mg/kg体重AS分别与50mg/kg体重ABZ、50mg/kg体重MBZ配伍用,平均减虫率为89.22%和97.55%,检虫数与75mg/kg体重ABZ、MBZ,30mg/kg体重AS单用药组比较,差异有统计学意义(P<0.05)。结论联合用药可降低给药剂量,并具有增效作用,50mg/kg体重TBD分别与50mg/kg体重MBZ、ABI配伍用对治疗大鼠华支睾吸虫病疗效较好。
Objective To observe the curative effect of TBD, AS, ABZ and MBZ in treating Clonorchis sinensis infection. Methods One hundred and fourteen rats were infected with Clonorchis sinensis, each with 100 cercariae. The rats were treated by gavage at the fifth week after infection. Infected rats were randomly divided into the same drug treatment group infected control group (each group of 5 to 10), the TBD, AS, ABZ and MBZ were administered according to high and low dose of single-dose and compatibility of two or two, Dayton, Observe the effect. Rats were sacrificed 10 days after the treatment. Anatomy, Clonorchis sinensis was collected in the bile duct and liver tissues. The average number of worms and the worm reduction rate were calculated. One-way ANOVA was performed on the average number of worms in the corresponding groups. Results After treated with 75 mg / kg body weight of TBD, MBZ and ABZ, the average worm reduction rates were 98.04%, 100% and 86.67%, respectively. After treatment with 30 mg / kg body weight of AS, the worm reduction rate was 87.25 %. When the dosage was reduced by 1/3, the average worm reduction rate of each drug treatment group dropped to 78.3%, 46.02%, 31.94% and 62.07% respectively. The average worm reduction rates of 50mg / kg body weight and 50mg / kg body weight MBZ, ABZ compatibility group were 98.43% and 100%, the number of sepsis and 75mg / kg body weight TBD, MBZ, ABZ single treatment group, the difference was statistically significant Significance (P <0.05). 20mg / kg body weight AS were respectively combined with 50mg / kg body weight ABZ and 50mg / kg body weight MBZ with the average worm reduction rate of 89.22% and 97.55%. The number of sewer was 75mg / kg body weight ABZ, MBZ, 30mg / kg body weight AS Compared with the medication group, the difference was statistically significant (P <0.05). Conclusions The combination therapy can reduce the dosage and has a synergistic effect. The combination of 50mg / kg body weight TBD with 50mg / kg body weight MBZ, ABI with good effect on the treatment of rat Clonorchiasis.