从两个番茄杂种Ｌ４０２、Ｌ４０４及它们的亲本共６份材料的叶片中提取ＤＮＡ，使用国产ＰＣＲ仪，建立了适合番茄ＲＡＰＤ分析的ＰＣＲ条件，进一步在１００个随机引物中找到了５个可用于鉴定这两个杂种纯度的引物，其中ＯＰＫ１４引物可同时用来鉴定两个杂种。利用该法鉴定杂种纯度不仅简单、迅速、可靠，而且因ＤＮＡ的用量少，不影响被检植株的后期生长。 DNA was extracted from leaves of 6 tomato hybrids L402, L404 and their parents, and PCR conditions suitable for tomato RAPD analysis were established by using a domestic PCR instrument. Five of the 100 random primers were used to identify Primers for the purity of these two hybrids were identified, where the OPK14 primer was used to identify both hybrids. Using this method to identify the hybrid purity is not only simple, rapid and reliable, but also because of the amount of DNA less, does not affect the late growth of the tested plants.