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目的:研究泥鳅多糖对小鼠脾细胞免疫应答的影响.方法:分别给正常小鼠、刀豆蛋白(ConA)或左旋咪唑免疫增强小鼠、环磷酰胺免疫抑制小鼠腹腔注射提取的泥鳅多糖,连续给药7d.用~H-胸苷掺入法测定T淋巴细胞的增殖.用~(51)Cr同位素标记法测定细胞毒T淋巴细胞的细胞毒性和天然杀伤细胞的活性.结果:泥鳅多糖5或10mg·kg~(-1)·d~(-1)可以提高T淋巴细胞的增殖,并增强细胞毒T淋巴细胞的细胞毒性和天然杀伤细胞的活性,还能增强ConA解除环磷酰胺对T淋巴细胞增殖抑制的能力,抑制率从环磷酰胺对照组的51.4％分别降低到18.2％和35.1％.而且,给予小鼠10或20mg·kg~(-1)·d~(-1)的泥鳅多糖,可以恢复环磷酰胺所致的天然杀伤细胞活性降低.结论:泥鳅多糖能增强小鼠脾细胞中T细胞、细胞毒T淋巴细胞和天然杀伤细胞的活性.
Objective: To study the effect of loach polysaccharides on the immune response of splenocytes in mice.Methods: The immunosuppressive mice were immunized with ConA or levamisole, , And administered for 7 days continuously.The proliferation of T lymphocytes was measured by ~ H-thymidine incorporation.The cytotoxicity of cytotoxic T lymphocytes and the activity of natural killer cells were measured by ~ (51) Cr isotope labeling.Results: Polysaccharide 5 or 10 mg · kg -1 d -1 could enhance the proliferation of T lymphocytes, enhance the cytotoxicity of cytotoxic T lymphocytes and the activity of natural killer cells, and also enhance the release of cyclic phosphorus The inhibitory rate of amide on T lymphocyte proliferation was reduced from 51.4% in cyclophosphamide control group to 18.2% and 35.1%, respectively. Moreover, the inhibitory rate of 10 or 20 mg · kg -1 · d ~ (-1) 1) could restore the natural killer cell activity induced by cyclophosphamide.Conclusion: Loach polysaccharide can enhance the activity of T cells, cytotoxic T lymphocytes and natural killer cells in mouse spleen cells.