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目的:探讨丹参酮(TⅡA)对癫痫大鼠认知功能障碍的治疗作用。方法:将24只成年雄性SD大鼠随机等分为健康对照组、模型组、TⅡA(每日10 mg/kg)治疗组。采用Morris水迷宫实验检测大鼠学习记忆功能,运用电生理技术在脑片水平检测海马CA1区长时程增强(LTP)。结果:(1)模型组大鼠在Morris水迷宫实验中寻找平台潜伏期明显长于健康对照组(P<0.05),TⅡA治疗组寻找平台潜伏期较模型组显著缩短(P<0.05)。空间探索试验中,模型组大鼠在目标象限的停留时间显著短于对照组(P<0.05),TⅡA治疗后大鼠在目标象限的停留时间较模型组明显延长(P<0.05)。(2)给予高频强直刺激(HFS)后各均可诱发LTP,且均持续1 h以上,与模型组比较缺氧组HFS刺激后LTP显著减弱(P<0.05),TⅡA治疗后LTP较模型组显著增强(P<0.05)。结论:TⅡA可显著改善癫痫大鼠认知功能障碍,该作用与TⅡA减轻癫痫大鼠海马LTP抑制有关。
Objective: To explore the therapeutic effect of tanshinone (TⅡA) on cognitive dysfunction in epileptic rats. Methods: Twenty-four adult male Sprague-Dawley rats were randomly divided into healthy control group, model group and TⅡA (10 mg / kg daily) treatment group. The Morris water maze test was used to detect the learning and memory function in rats. The long-term potentiation (LTP) of hippocampal CA1 region was detected by electrophysiological technique. Results: (1) The latent period of platform searching for Morris water maze test in model group was significantly longer than that in healthy control group (P <0.05). The latent period of finding platform in TⅡA treatment group was significantly shorter than that in model group (P <0.05). In the spatial exploration test, the retention time in the target quadrant of the model group was significantly shorter than that in the control group (P <0.05). The retention time in the target quadrant of the model rats after TIIA treatment was significantly longer than that in the model group (P <0.05). (2) LTP was induced in all rats after high frequency tonic stimulation (HFS) for more than 1 h, compared with model group, LTP in Hypoxia group was significantly decreased (P <0.05) Group was significantly enhanced (P <0.05). Conclusion: TⅡA can significantly improve the cognitive dysfunction in epileptic rats, which may be related to the effect of TⅡA on the inhibition of LTP in the hippocampus of epileptic rats.