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目的:检测Omp31-BLS融合肽抗血清的质量。方法:利用间接酶联免疫吸附实验、免疫双扩散实验以及凝集实验检测Omp31-BLS融合肽制备的抗血清的质量。结果:Omp31-BLS融合肽抗血清可以较好地识别Omp31-BLS融合肽蛋白质,而且,当抗体被稀释4 000倍的时候,酶联免疫吸附实验测定的OD值为1.2,明显高于其他3个对照组。在免疫双扩散实验中,抗血清被稀释64倍的时候,仍然能够出现明显的免疫沉淀线。此外,凝集实验也反映出Omp31-BLS融合肽抗血清可以与Omp31-BLS融合肽抗原发生强烈的凝集作用。结论:Omp31-BLS融合肽抗血清可以较好地识别对应的蛋白质抗原,二者之间存在明显的抗原-抗体反应,为利用Omp31-BLS融合肽进行布鲁氏菌病的血清学诊断提供了理论基础和技术支持。
Objective: To detect the quality of Omp31-BLS fusion peptide antiserum. Methods: The quality of antiserum prepared by Omp31-BLS fusion peptide was detected by indirect enzyme-linked immunosorbent assay (ELISA), double immunodiffusion assay and agglutination test. Results: Omp31-BLS fusion peptide antiserum can recognize Omp31-BLS fusion peptide protein better, and when the antibody was diluted 4 000 times, the OD value determined by enzyme-linked immunosorbent assay was 1.2, which was significantly higher than the other 3 A control group. In immunodouble diffusion experiments, antisera were still able to appear clear immunoprecipitation lines when diluted 64-fold. In addition, agglutination experiments also showed that Omp31-BLS fusion peptide antiserum can strongly agglutinate with Omp31-BLS fusion peptide antigen. CONCLUSION: Omp31-BLS fusion peptide antiserum can recognize the corresponding protein antigen well, and there is a significant antigen-antibody reaction between the two. This provides the basis for serological diagnosis of brucellosis using Omp31-BLS fusion peptide Theoretical basis and technical support.