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目的研究太原市手足口病(Hand,Foot and Mouth Disease,HFMD)患者中,肠道病毒A71型(Enterovirus Type A71,EVA71)的分子进化特征。方法采集太原市HFMD患者的咽拭子和粪便标本,采用荧光定量逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)方法检测EV核酸。然后随机选取11株EVA71阳性标本进行衣壳蛋白(Capsid Protein)VP1编码区基因扩增、核苷酸序列测定和分析,与其他48株各基因型和基因亚型的EV71代表株构建基因亲缘性关系树。结果太原市11株EVA71均属于C4基因亚型C4a进化分支,但11株EVA71在VP1编码区核苷酸和氨基酸的同源性分别为95.7%~100.0%和99.0%~100.0%。在亲缘进化树中显示,这些EVA71处在不同的簇中,表现为11株EVA71至少属于3条病毒传播链。结论太原市流行的EVA71属于C4基因亚型C4a进化分支,并且存在多个传播链。与中国其他地区流行的EVA71在基因亲缘关系和流行时间关系上都很接近,说明太原市EVA71不是独立进化的,而是与中国其他地区流行的EVA71在共同进化。
Objective To study the molecular evolution of Enterovirus Type A71 (EVA71) in patients with Hand, Foot and Mouth Disease (HFMD) in Taiyuan. Methods Throat swabs and stool samples were collected from patients with HFMD in Taiyuan City. The EV nucleic acids were detected by reverse transcription-polymerase chain reaction (RT-PCR). Then, 11 EV71 positive samples were randomly selected to amplify the capsid protein VP1 coding region and to determine the nucleotide sequence. The results were compared with those of 48 EV71 isolates with different genotypes and subtypes Relationship tree. Results All of the 11 strains of EVA71 in Taiyuan belonged to the C4a evolutionary branch of C4 gene. However, the nucleotide and amino acid identities of 11 strains of EVA71 in the VP1 coding region were 95.7% -100.0% and 99.0% -100.0%, respectively. In the phylogenetic tree, these EVA71s are in different clusters, showing that at least 11 strains of EVA71 belong to at least 3 virus transmission chains. Conclusion The prevalence of EVA71 in Taiyuan belongs to the evolutionary branch of C4 subtype C4a, and there are multiple transmission chains. Similar to the prevalence of EVA71 in other regions of China, the genetic relationship and prevalence of EVA71 are very similar, indicating that the EVA71 in Taiyuan is not evolving independently but co-evolving with the popular EVA71 in other parts of China.