论文部分内容阅读
以某电机厂电焊尘采集物为受试物(受试物浓度分别为100.00μg/ml、200.00μg/ml、300.00μg/ml、400.00μg/ml、500.00μg/ml、0.00μg/ml),用噻唑蓝(MTT)染色的方法检验作用12 h、24 h、36 h的细胞相对存活率;单细胞凝胶电泳技术检测作用2 h、4 h、8 h的细胞DNA损伤情况。结果显示,随着染毒时间的延长和染毒剂量的增加,细胞存活率下降,在浓度为500.00μg/ml的颗粒物作用细胞36 h后,细胞的存活率仅为19.02%;电焊尘颗粒物的各浓度组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组,差异有统计学意义(P<0.05)。提示电焊尘颗粒物可以降低哺乳动物细胞的相对存活率,并导致NIH/3T3细胞不同程度的DNA损伤。
The electric welding dust collected from a motor factory was used as the test substance (the concentration of the test substance was 100.00μg / ml, 200.00μg / ml, 300.00μg / ml, 400.00μg / ml, 500.00μg / ml, 0.00μg / ml respectively) The cell viability at 12 h, 24 h, 36 h was examined by MTT staining. The DNA damage was detected by single cell gel electrophoresis at 2 h, 4 h, 8 h. The results showed that the survival rate of cells decreased with the prolongation of exposure time and dose of irradiation, and the survival rate was only 19.02% at the concentration of 500.00μg / ml for 36 hours. The tailing rate and tail DNA content induced by different concentration groups were higher than that of the negative control group at each time point, the difference was statistically significant (P <0.05). Tip electric dust particles can reduce the relative survival of mammalian cells, and lead to NIH / 3T3 cells to varying degrees of DNA damage.