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目的:观察没食子提取物(MSZ)是否具有诱导癌细胞凋亡的作用。方法:人乳腺癌细胞Bcap-37经不同浓度MSZ作用24~48h后,经倒置显微镜观察细胞形态的变化,流式细胞仪检测细胞凋亡周期及凋亡率。结果:在倒置显微镜下,可见大量具有凋亡特征的细胞;流式细胞仪检测出现明显的凋亡峰,呈剂量依赖性。结论:MSZ具有抑制乳腺癌细胞Bcap-37生长,诱导其凋亡的作用。
OBJECTIVE: To observe whether gallate extract (MSZ) has the effect of inducing apoptosis in cancer cells. METHODS: Human breast cancer cell line Bcap-37 was treated with different concentrations of MSZ for 24 to 48 h. Changes in cell morphology were observed by inverted microscope. Apoptosis cycle and apoptosis rate were measured by flow cytometry. RESULTS: Under the inverted microscope, a large number of cells with apoptotic characteristics were seen; flow cytometry showed a clear apoptotic peak in a dose-dependent manner. Conclusion: MSZ can inhibit the growth of breast cancer cell Bcap-37 and induce its apoptosis.