论文部分内容阅读
目的:分析1例免疫缺陷患儿排出Ⅱ型疫苗衍生脊髓灰质炎病毒病原学检测结果及对当地维持无脊灰的影响。方法:对免疫缺陷患儿粪便标本及其密切接触者粪便标本和污水标本采用病毒分离的方法,对分离到的Ⅱ型脊灰病毒进行VP1编码区基因核苷酸序列测定和分析。结果:2011年在贵州省1例AFP病例双份粪便标本中,分离到2株Ⅱ型脊灰病毒(PV),经核苷酸序列测定和分析鉴定为Ⅱ型VDPVs,临床诊断为原发性免疫缺陷病,患儿曾接种过4剂口服脊灰减毒活疫苗(OPV)。对该患儿连续采集粪便标本检测,未再发现VDPV。贵州省AFP病例分类专家诊断小组诊断为周期性麻痹、VDPV携带者。结论:此次发现的Ⅱ型VDPV发生在OPV高免疫覆盖率地区,未发现形成VDPV循环的证据。
OBJECTIVE: To analyze the etiological test results of type I vaccine-derived poliovirus (EPV) eradication in one immunocompromised child and its effect on poliovirus maintenance in our country. Methods: The nucleotide sequences of the VP1 coding region of type Ⅱ poliovirus isolated and isolated from feces specimens and their close contact with immunodeficiency children were determined by virus isolation method. Results: Two strains of poliovirus type Ⅱ (PV) were isolated from one stool specimen from a case of AFP in Guizhou Province in 2011, and were identified as type VDPVs by nucleotide sequence analysis and analysis. The clinical diagnosis was primary Immune deficiency disease, children have been vaccinated 4 oral live attenuated polio vaccine (OPV). Continuous detection of stool samples in this child, no further discovery of VDPV. Guizhou province AFP case classification expert diagnosis group diagnosed as periodic paralysis, VDPV carriers. Conclusion: The type Ⅱ VDPV found in this study occurred in areas with high immunocompetence of OPV. No evidence of VDPV circulation was found.