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目的:研究Notch1、Jagged1和COX-2在胃癌组织中的表达情况,分析其与临床病理特征及Hp感染的关系。方法:应用免疫组织化学S-P法,采用组织芯片技术,检测60例胃癌组织、60例癌旁组织及20例正常胃黏膜组织中Notch1、Jagged1和COX-2蛋白的表达;采用Warthin-starry染色法检测各组织中Hp感染状况。结果:Notch1与Jagged1在胃癌组中阳性表达率(40.0%,70.0%)低于癌旁组(81.7%,96.7%)和正常组(85.0%,100.0%),差异有统计学意义(P<0.05)COX-2在胃癌组中的阳性表达率(60.0%)高于癌旁组(35.0%)和正常组(0),差异有统计学意义(P<0.05)。Notch1和Jagged1与胃癌的临床病理特征均无关(P>0.05)。COX-2的表达与胃癌的分化程度、浸润深度(P=0.013,P=0.013)有关。Notch1与Jagged1在胃癌组中的表达呈正相关(r=0.460,P<0.005);Notch1与COX-2表达呈负相关(r=-0.314,P>0.05);Jagged1与COX-2表达无相关性(r=-0.175,P>0.05)。Hp在胃癌组中阳性感染率低于癌旁组(76.6%,91.7%),差异有统计学意义(P<0.05)。Notch1、Jagged1和COX-2在胃癌组中Hp感染阳性与Hp感染阴性的阳性表达率相比,差异无统计学意义(均P>0.05)。胃癌组织中Notch1、Jagged1和COX-2的表达与Hp感染率无关(P>0.05)。结论:胃癌中存在Notch信号通路的异常,Notch1和Jagged1的低表达可能导致COX-2的高表达,最终导致胃癌的发生发展;Hp感染可能在早期胃癌的发生中起重要作用。
Objective: To investigate the expression of Notch1, Jagged1 and COX-2 in gastric cancer and to analyze the relationship between Notch1, clinicopathological features and Hp infection. Methods: The expressions of Notch1, Jagged1 and COX-2 protein in 60 cases of gastric cancer tissue, 60 cases of paracancerous tissues and 20 cases of normal gastric mucosa tissues were detected by immunohistochemical SP method and tissue microarray. Warthin-starry staining Hp infection in each tissue was examined. Results: The positive expression rates of Notch1 and Jagged1 in gastric cancer group were lower than those in paracancer group (81.7%, 96.7%) and normal group (85.0%, 100.0%) (40.0%, 70.0%, P < 0.05). The positive expression rate of COX-2 in gastric cancer group (60.0%) was higher than that in adjacent cancer group (35.0%) and normal group (0), the difference was statistically significant (P <0.05). Notch1 and Jagged1 had no correlation with the clinicopathological features of gastric cancer (P> 0.05). The expression of COX-2 was correlated with the degree of differentiation and depth of invasion (P = 0.013, P = 0.013). The expression of Notch1 and Jagged1 in gastric cancer group was positively correlated (r = 0.460, P <0.005). The expression of Notch1 and COX-2 was negatively correlated (r = -0.314, P> 0.05) (r = -0.175, P> 0.05). The positive rate of Hp in gastric cancer group was lower than that in adjacent cancer group (76.6%, 91.7%), the difference was statistically significant (P <0.05). There was no significant difference in the positive expression rate of Notch1, Jagged1 and COX-2 in gastric cancer between Hp infection and Hp infection (all P> 0.05). The expression of Notch1, Jagged1 and COX-2 in gastric cancer tissue was not related to the infection rate of Hp (P> 0.05). Conclusion: The abnormal Notch signaling pathway exists in gastric cancer. The low expression of Notch1 and Jagged1 may lead to the high expression of COX-2, eventually leading to the development of gastric cancer. Hp infection may play an important role in the development of early gastric cancer.