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目的复制四氧化二氮(N2O4)吸入致肺损伤动物模型,检测心钠素(ANP)、高铁血红蛋白(MetHb)和肺病理学变化,研究其肺损伤机制。方法实验动物为美国癌症研究所(ICR)小鼠16只,随机分成正常组和中毒组,每组8只。分时相(24 h、48 h、72 h)取血测定ANP和MetHb含量,同期进行肺病理学检查。结果 N2O4中毒后,各组动物均出现肺水肿,同期血清ANP含量呈进行性升高,全血MetHb也较正常组显著增高。结论测定ANP可以及时反映N2O4中毒后发生肺水肿伤情变化,MetHb也在其中扮演了重要角色,实时监测二者含量可用于指导临床救治。
OBJECTIVE: To study the mechanism of lung injury in animal models of lung injury induced by inhalation of N2O4, to detect the changes of ANP, Methemoglobin (HHb) and lung pathology. Methods Sixteen mice from the American Cancer Institute (ICR) were randomly divided into normal group and poisoning group, with 8 mice in each group. Time-phase (24 h, 48 h, 72 h) blood was collected to determine the content of ANP and MetHb, the same period for lung pathology. Results After N2O4 poisoning, pulmonary edema was observed in all the animals. The serum ANP level increased progressively and the whole blood MetHb level was significantly higher than that of the normal group. Conclusion The determination of ANP can reflect the changes of pulmonary edema after N2O4 poisoning in time, and MetHb also plays an important role in the pathogenesis of N2O4 poisoning. The content of both can be used to guide clinical treatment.