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探讨重组FN多肽CH50对IFN-γ基因转染癌细胞体内生长及免疫刺激作用的影响。将小鼠IFN-γ基因转染黑色素瘤B16/Fl细胞,测定其表达产物与CH50协同刺激巨噬细胞产生NO的作用、转染细胞接种小鼠并注射CH50时对脾细胞的免疫刺激作用及瘤细胞的体内生长特性。结果表明,转染细胞表达产物可与CH50协同作用刺激巨噬细胞产生NO。转染细胞表达IFN-γ水平较低,仍可在体内形成肿瘤,注射CH50能够抑制其形成肿瘤。基因转染细胞和CH50能够促进脾细胞对肿瘤细胞的杀伤能力。研究表明,CH50不仅可以提高肿瘤疫苗的安全性,也可以提高肿瘤疫苗刺激机体免疫系统的作用。并提示,表达CH50和IFN-γ双因子的肿瘤疫苗可为提高肿瘤疫苗治疗肿瘤的效果开辟新的途径。
To investigate the effect of recombinant FN polypeptide CH50 on the growth and immune stimulatory effect of IFN-γ gene transfected cancer cells. The mouse IFN-γ gene was transfected into melanoma B16/F1 cells, and the expression product and CH50 were co-stimulated to produce NO by macrophages, and the immune stimulatory effect on spleen cells when mice were transfected with cells and injected with CH50. In vivo growth characteristics of tumor cells. The results showed that the transfected cell expression product could synergize with CH50 to stimulate macrophage production of NO. Transfected cells express low levels of IFN-γ and can still form tumors in vivo. Injection of CH50 can inhibit the formation of tumors. Gene transfection of cells and CH50 can promote the killing ability of spleen cells to tumor cells. Studies have shown that CH50 can not only improve the safety of cancer vaccines, but also improve the role of tumor vaccines in stimulating the body’s immune system. It is also suggested that tumor vaccines expressing the dual factor of CH50 and IFN-γ can open a new way to improve the efficacy of tumor vaccines in the treatment of tumors.