:研究中华鳖胚胎细胞体外培养适宜培养渗透压、培养基和培养温度等条件 ,并进行传代试验。结果表明 ,胚胎原代细胞在 2 5～ 2 8℃ ,渗透压 2 50mmol/kg ,RPMI - 16 4 0 +10 %小牛血清培养液及无CO2 的条件下 ,生长良好 ,72h长满单层 ,并能顺利传代。细胞动力学试验表明 :传代细胞 1～ 3代为适应期、4～ 6代为旺盛期、7～ 9代为衰退期。第 6代细胞染色体组型分析 ,其染色体为整二倍体 ,并未发生转化 : To study the conditions of in vitro culture of Trionyx sinensis embryos cultured in vitro, such as osmotic pressure, culture medium and culture temperature, and to conduct the passage test. The results showed that the embryonic primary cells grew well at 25 ℃ and 28 ℃ with the osmotic pressure of 250mmol / kg, RPMI - 1640 + 10% calf serum medium and without CO2, , And can pass on smoothly. The results of cell kinetics showed that the passages 1 to 3 of the passage cells were acclimatization period, the stage of 4-6 generations was vigorous, and the stage of 7-9 was the decay stage. Genomic analysis of the 6th generation showed that the chromosome was whole diploid and did not transform