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目的探讨α L 岩藻糖苷酶 (AFU) ,甲胎蛋白 (AFP)及其异质体 (AFP L3)在原发性肝癌诊断中的意义。方法设定原发性肝癌(PHC)组 ,慢性肝炎肝硬变组 ,正常对照组 ,测定各组的AFU、AFP及AFP L3。AFU采用比色法 ,AFP采用电化学发光法 ,AFP L3采用扁豆凝集素 (LCA)亲合电泳印迹法。结果在 1 0 0例PHC患者中 ,AFP >2 0ng/ml的阳性率为 68.0 % ,AFU阳性率为 76 .0 % ,AFP L3阳性率为 78.0 %。AFP <2 0ng/ml的 2 0例PHC患者中 ,AFU阳性率为 70 .0 % ,AFP L3阳性率为 1 0 .0 % ;AFP在 2 0～ 40 0ng/ml的 30例PHC患者中 ,AFU阳性率为 80 .0 % ,AFP L3阳性率为 90 .0 % ;AFP >40 0ng/ml的 50例PHC患者中 ,AFU阳性率为 76 .0 % ,AFP L3阳性率为98.0 % ,三者联合应用的PHC患者中阳性者 91例 ,阳性率为 91 .0 % ,三者同时阳性者 55例 ,阳性率为 55 .0 %。结论AFU、AFP L3联合检测在AFP <40 0ng/ml的PHC患者中可优势互补 ,阳性率显著提高 ,尤其是对小肝癌的早期诊断及其与良性肝病的鉴别诊断具有重要价值
Objective To investigate the significance of α-L-fucosidase (AFU), AFP and AFP L3 in the diagnosis of primary liver cancer. Methods Primary liver cancer (PHC) group, chronic hepatitis cirrhosis group and normal control group were set up. AFU, AFP and AFP L3 in each group were determined. AFU using colorimetric method, AFP using electrochemiluminescence, AFP L3 lentil lectin (LCA) affinity electrophoresis. Results In 100 PHC patients, the positive rate of AFP> 20ng / ml was 68.0%, the positive rate of AFU was 76.0%, and the positive rate of AFP L3 was 78.0%. In 20 PHC patients with AFP <20 ng / ml, the positive rate of AFU was 70.0% and the positive rate of AFP L3 was 1.0%. Among 30 PHC patients with AFP ranging from 20 to 40 0 ng / ml, AFU positive rate was 80.0%, AFP L3 positive rate was 90.0%; AFP> 40 0ng / ml in 50 PHC patients, AFU positive rate was 76.0%, AFP L3 positive rate was 98.0%, three Among the PHC patients, 91 cases were positive, the positive rate was 91.0%. 55 cases were positive at the same time and the positive rate was 55.0%. Conclusion The combined detection of AFU and AFP L3 in the PHC patients with AFP <40 0ng / ml may be complementary to each other and the positive rate is significantly increased, especially for the early diagnosis of small hepatocellular carcinoma and its differential diagnosis with benign liver disease