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目的研究染矽尘大鼠支气管肺泡灌洗液(BALF)中炎症细胞成分、超氧化物歧化酶(SOD)活力及可溶性蛋白成分、微量元素成分。方法采用气管非暴露法建立大鼠矽肺模型,在体全肺肺灌洗法收集支气管肺泡灌洗液。对BALF中的细胞进行总数计数及分类计数;分别采用黄嘌呤氧物酶法、Bradford法、电感耦合等离子体原子发射光谱法(ICP-AES),对BALF中的SOD活力、总蛋白含量、微量元素进行测定;采用聚丙烯酰胺凝胶电泳法分离BALF可溶性蛋白;同时对肺组织进行HE染色,普通光镜下观察肺组织的病理学改变。结果实验组较对照组BALF中细胞总数及各类炎性细胞数均明显升高(P<0.05);SOD活力及蛋白总量和条带均见增多(P<0.05);几种微量元素含量差异也有统计学意义,如铁含量降低,锌、锰含量升高。肺组织病理学观察发现染矽尘大鼠肺组织出现严重的病理学改变。结论本实验发现大鼠染矽尘后BALF中有形成分(细胞)与某些无形成分出现明显改变(P<0.05),均不同程度地影响了尘肺的发生及发展变化过程。
Objective To study the inflammatory cytokines, superoxide dismutase (SOD) and soluble protein and trace element in bronchoalveolar lavage fluid (BALF) of rats exposed to silica dust. Methods The rat model of silicosis was established by tracheal non-exposure method, and bronchoalveolar lavage fluid was collected by whole lung lung lavage method. BALF in the total number of cells count and classification of count; respectively using xanthine oxidase method, Bradford method, inductively coupled plasma atomic emission spectrometry (ICP-AES), the BALF SOD activity, total protein content, trace Element was measured. The soluble protein of BALF was isolated by polyacrylamide gel electrophoresis. At the same time, the lung tissue was stained with HE and the pathological changes of lung tissue were observed under ordinary light microscope. Results Compared with the control group, the total number of cells and the number of inflammatory cells in BALF were significantly increased in the experimental group (P <0.05), while the activity of SOD and the total amount of proteins and bands increased (P <0.05). The contents of several trace elements The difference was also statistically significant, such as reduced iron content, zinc, manganese content increased. Pulmonary histopathology showed that the pathological changes of the lung tissue in rats exposed to silica were serious. Conclusions In this experiment, we found that there was a significant change in the shape components (cells) and some invisible components of BALF in rats exposed to silica dust (P <0.05), which all affected the occurrence and development of pneumoconiosis to varying degrees.