Objective: To explore the reference value of intraoperative ultrasonography in the operative procedure and postoperative treatment with relation to hepatopancreatobiliary surgery.Methods: 103 cases of hepatopancreatobiliary operations were collected in our hospital from January 1996 to October 2005, in which 68 cases were males and the others were females, age ranged from 45 to 76 years.Hepatocellular carcinoma were 26 cases, gallbladder carcinoma were 5 cases, cholangiocarcinoma were 6 cases, pancreatic carcinoma were 10 cases and other 56 cases were gall stone and/or choledocholithiasis.Intraoperative ultrasonography with high frequency probe was used in all cases.Results: Some minimal occult focuses was found by intraoperative ultrasonography.We found vascular tumor thrombosis in 70% cases of hepatocellular carcinomas.One case was diagnosed as pancreatic carcinoma before operation and found choledochal tract dilatation concurrently with cholangitis by intraoperetive ultrasonography.Eight cases of gallstones with no choledochal stones before operation were found by intraoperative ultrasonography that stone lain in low segment of common bile duct.Conclusion: The image data of high quality obtained by intraoperative ultrasonography could not only direct our more proper operative practice but also favor us to make more reasonable postoperative treatment plan.