Puerarin is a natural isolfavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puer-arin (8-(β-D-Glucopyranosyl-7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses signiifcantly up-regulated the expression of growth-associated protein 43 in the L4-6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atro-phy of the triceps surae on the affected side and promoted the regeneration of nerve ifbers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciat-ic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.