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以吲哚乙酸(IAA)为模板分子、乙腈为致孔剂,采用沉淀聚合法制备了其分子印迹聚合物,通过静态吸附实验研究了致孔剂乙腈的用量及4-乙烯基吡啶(4-VP)和丙烯酰胺(AA)两种不同性质的功能单体(M)对印迹聚合物吸附性能的影响。结果表明,以4-VP为M,乙腈的加入量分别为20、25、37.5和50 m L时,制备的印迹聚合物P1~P4均具有良好的分散性,且聚合物P4对IAA具有最显著的印迹效应(印迹因子IF=2.92);而在同样条件下以AA为M的印迹聚合物的印迹效应较小(IF=1.73)。选择性实验表明,聚合物P4能够从吲哚羧酸同系物中选择性结合IAA。Scatchard分析表明,聚合物P4对IAA表现出两类结合位点,其高亲和力与低亲和力结合位点的平衡离解常数分别为Kd1=0.798 mmol/L和Kd2=7.76 mmol/L,对应的最大结合量分别为21.0和90.7μmol/g。
Indoleacetic acid (IAA) was used as a template molecule and acetonitrile was used as a porogen. The molecularly imprinted polymers were prepared by precipitation polymerization. The amount of acetonitrile used in the porogen was measured by static adsorption experiments. VP) and acrylamide (AA) two different properties of the functional monomer (M) on the adsorption properties of imprinted polymers. The results showed that the P1 ~ P4 polymers prepared with the 4-VP of M and acetonitrile at 20, 25, 37.5 and 50 m L had good dispersibility, and the polymer P4 had the highest IAA Significant imprinting effect (imprinting factor IF = 2.92); imprinting effect of imprinted polymer with AA as M under the same conditions was smaller (IF = 1.73). Selective experiments show that polymer P4 is able to selectively bind IAA from indole carboxylic acid homologues. Scatchard analysis showed that polymer P4 exhibited two types of binding sites for IAA, and the equilibrium dissociation constants of high-affinity and low-affinity binding sites were Kd1 = 0.798 mmol / L and Kd2 = 7.76 mmol / L, respectively, corresponding to the maximum binding The quantities were 21.0 and 90.7 μmol / g, respectively.