作者报导5例经临床、实验室检查和肝活检证实为酒精性肝硬变患者腹水中的细胞学及细胞遗传学检查结果。将腹水离心后的沉渣涂片用Papanico-laou、Giemsa、阶段性希夫酸和Alcian蓝方法染色后在光镜下检查。至少取20ml腹水液离心,将细胞团再浮悬在5ml 199混合液中,加入浓度为5μg/ml的秋水仙素,浮悬液在37℃孵育2小时。所获细胞用标准方法制备后作染色体分析。短期培养可增加中期 The authors report cytology and cytogenetic findings in 5 ascites confirmed by clinical, laboratory and liver biopsy in patients with alcoholic cirrhosis. The ascites centrifugal sediment smears were stained with Papanico-laou, Giemsa, staged Schiff acid and Alcian blue methods and examined under the light microscope. At least 20 ml of ascites fluid is centrifuged, the cell pellet is resuspended in 5 ml of 199 mixture, colchicine is added at a concentration of 5 μg / ml, and the suspension is incubated at 37 ° C for 2 hours. The cells obtained were analyzed for chromosomal analysis by standard methods. Short-term training can increase the medium-term