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用喉癌抗原免疫ＢＡＬＢ／Ｃ小鼠的脾细胞与ＮＳ－１小鼠骨髓瘤细胞融合，经筛选获得４株分泌抗人喉癌单克隆抗体（ＭｃＡｂ）的杂交瘤细胞株，均收集到腹水型ＭｃＡｂ，并验证其滴度高，纯化效果好，特异性强。用其中３株ＭｃＡｂ混合，对１２４例喉癌及４０例正常人血清予以检测，结果喉癌患者血清中喉癌相关抗原水平明显高于对照组，Ｐ＜０．０１，且随病情的加重，喉癌相关抗原水平呈上升趋势。故本室建立的抗人喉癌混合ＭｃＡｂ可作为一种较为理想的肿瘤标记物，为喉癌的诊断、病情监测、预后判定提供了一种敏感、特异的测试手段。
The spleen cells of BALB / C mice immunized with laryngeal cancer antigen were fused with NS-1 mouse myeloma cells. Four hybridoma cell lines secreting anti-human laryngeal carcinoma monoclonal antibody (McAb) were screened, and all the ascites Type McAb, and verify its high titer, purification, good specificity. Using three McAbs mixed, 124 cases of laryngeal cancer and 40 cases of normal serum were detected, the results of laryngeal cancer patients serum laryngeal cancer antigen levels were significantly higher than the control group, P <0.01, and with the severity of the disease, Laryngeal cancer-related antigen levels are on the rise. Therefore, the anti-human laryngeal mixed McAb can be used as a more ideal tumor marker for the diagnosis of laryngeal cancer, disease monitoring, prognosis provides a sensitive and specific test means.