Autologous adipose tissue is one of the most commonly used fillers. Plastic surgeons are increasingly using it in aesthetic or reconstructive surgery owing to its abundant source, easy availability, and minimal invasiveness. Increasing amount of evidence has shown its reparative ability rather than just filling utility due to the presence of adipose-derived stem cells (ADSCs) that exhibit multipotential differentiation and paracrine characteristics. However, traditional enzymatic isolation of ADSCs is often difficult in clinical settings, limiting its application. Recently, with the development of mechanical techniques for preparation of lipoaspirates, bioactive adipose tissue can be successfully identified and isolated. Such components with therapeutic potential might also be helpful in tissue repair and regeneration. In this article, we introduced several types of mechanized lipoaspirates derivatives (such as emulsified fat, adipose extracellular matrix (ECM), and cell-free extraction) and their applications from bench to bedside.