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目的 观察脑室注射高渗氯化钠诱导的脑内c-fos 与egr-1 的表达特征,并用双重细胞染色方法观察脑内c-fos 表达与下丘脑视上核(SON)中加压素(AVP) 能神经元之间的关系。方法 用SD 种系清醒大鼠,侧脑室微量注射高渗氯化钠,1h 后作c-fos 与egr- 1 的免疫细胞化学染色和细胞双重染色。结果 (1) 脑室注射高渗氯化钠可在终末血管器官(OVLT)、正中视前核( MPN) 、SON、下丘脑室旁核(PVN) 以及后脑的最后区(AP) 、孤束核(STN) 和臂旁外侧核(LPBN) 中同时诱导c-fos 和egr- 1 表达,在同一区域内egr- 1 的表达略低于c-fos,但无统计学差异;(2) 穹窿下器官(SFO)中均无c-fos 和egr-1 表达;(3)SON 中可见部分AVP免疫反应阳性与c- fos表达共存于同一神经元。结论 c-fos 和egr-1 对脑内高渗刺激诱导的表达部位基本一致,进一步证实了以往的研究结果。另外,脑内注射高渗氯化钠诱导的下丘脑细胞活动至少部分是通过AVP能神经元的。
Objective To observe the expression of c-fos and egr-1 in rat brain induced by hypertonic sodium chloride and observe the expression of c-fos and vasopressin in hypothalamic supraoptic nucleus (SON) by double cell staining AVP) can be the relationship between neurons. Methods SD SD rats were sensitized with sodium chloride injection into the lateral ventricle. After 1 h, immunocytochemistry and double staining of c-fos and egr-1 were performed. Results (1) Intracerebroventricular injection of hypertonic sodium chloride can induce apoptosis in terminal vascular organs (OVLT), MPN, SON, paraventricular nucleus (PVN) of the hypothalamus, The expressions of c-fos and egr-1 in the nucleus (STN) and the paralateral arm nucleus (LPBN) were also observed. The expression of egr-1 in the same region was slightly lower than that in c-fos There was no c-fos and egr-1 expression in the lower organ (SFO). (3) Some AVP immunoreactive positive and c-fos expression coexisted in the same neuron in SON. Conclusions The expressions of c-fos and egr-1 in the brain induced by hypertonic stimulation are basically the same, which further confirm the previous findings. In addition, intracerebral injection of hypertonic sodium chloride induces hypothalamic cell activity at least partially through AVP neurons.