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Objective:To explore antischitosome effects of artemether,hemin and Fe on S/LDH.Methods: Enzyme activity of rS/LDH was assayed in the standard reaction system by adding different concentration of reagents(0.00-0.10 mM artemether,0.00-0.02 mM hemin,0.00-0.50 mM Fe~(3+)). Same solvents of the each reagent were used as control.Results:There was no enzyme activity inhibition observed at 0.10 mM artemther:obivious inhibition for lactate oxidation reaction and pyruvate reduction reaction were detected at 0.002 mM and 0.004 mM of hemin,respectively: comparing with that of the control(P<0.05).The relative enzymatic activity inhibitions for pyruvate reduction reaction and lactate oxidation reaction at 0.02 mM hemin were 93.48%and 100.00%,respectively,comparing with that of the control(P<0.01):both pyruvate reduction and lactate oxidation reaction were inhibited completely at 0.50 mM Fe~(3+),comparing with that of the control(P<0.01).Conclusions:The results implied that SjLDH was not the direct molecular target of artemether.Hemin and Fe are inhibitors of SjLDH.
Objective: To explore antischitosome effects of artemether, hemin and Fe on S / LDH. Methods: Enzyme activity of rS / LDH was assayed in the standard reaction system by adding different concentration of reagents (0.00-0.10 mM artemether, 0.00-0.02 mM hemin , 0.00-0.50 mM Fe ~ (3+)). Same solvents of the each reagent were used as control. Results: There was no enzyme activity inhibition observed at 0.10 mM artemther: obivious inhibition for lactate oxidation reaction and pyruvate reduction reaction were detected at 0.002 mM and 0.004 mM of hemin, respectively: comparing with that of the control (P <0.05). The relative enzymatic activity inhibitions for pyruvate reduction reaction and lactate oxidation reaction at 0.02 mM hemin were 93.48% and 100.00% respectively, comparing, comparing with that of the control with that of the control (both P <0.01): both pyruvate reduction and lactate oxidation reaction were inhibited completely at 0.50 mM Fe ~ (3 +), comparing with that of the control (P <0.01) .Conclusions: The results implied that SjLDH was not the direct molecular target of artemether. Hemin and Fe are inhibitors of SjLDH.