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目的:建立对荭草提取物血清药物化学研究方法,探讨荭草提取物的药效物质基础。方法:采用血清药物化学研究方法,在建立荭草提取物超高效液相(UPLC)指纹图谱的基础上,通过比较荭草提取物、荭草给药后含药血清、空白血清UPLC指纹图谱,找到大鼠灌胃荭草提取物后不经代谢的原型物质。结果:大鼠灌胃荭草提取物后,从含药血清中发现了18个代谢产物,其中1个为大鼠空白血清中存在的内源性物质,5个为荭草提取物中原型吸收入血的成分,其余成分可能为原型成分在体内的代谢产物。结论:应用UPLC建立快速测定大鼠灌胃荭草提取物后血清药物成分,其中17个代谢产物可能为荭草口服吸收后在体内的代谢产物,对其深入研究将为建立荭草谱效关系模型,确定荭草的药效物质与代谢作用机制奠定基础。
OBJECTIVE: To establish a method for the study of serum medicinal chemistry of extract of Hedyotis diffusa. Methods: Based on the UPLC fingerprinting method of UPLC, the UPLC fingerprinting of the extracts from the genus Rubus and the extracts from the genus Rubra were analyzed by serum chemistry and chemical methods. Find rat gavage extract without metabolism after the prototype material. Results: After the rats were fed with the extract of the stomach grass, 18 metabolites were found from the drug-containing serum, of which 1 was the endogenous substance present in the blank serum of the rats and 5 were the prototype absorbed in the extract of the hay fever Blood composition, the remaining components may be the prototype components in the body metabolites. Conclusion: UPLC was used to establish a rapid determination of serum drug composition in rats after Radix Ophiopogon japonicus extract, of which 17 metabolites may be the metabolites in vivo after the oral absorption of Radix Rehmanniae, and its further study will establish a spectrum- Model, to determine the pharmacological effects of the material and metabolic mechanisms lay foundation.