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目的:检测DNA聚合酶■(Pol■)在膀胱肿瘤细胞株及移行细胞癌组织中的表达,探讨Pol■在移行细胞癌组织中表达的意义。方法:培养膀胱肿瘤细胞株BIU87细胞、T24细胞株,利用RT-PCR方法检测Pol■在膀胱肿瘤细胞株中的表达;收集人膀胱粘膜正常组织、临床膀胱肿瘤和肾盂癌的移行细胞癌组织标本,利用RT-PCR方法检测组织标本中Pol■的表达。结果:Pol■在膀胱肿瘤细胞株中丰富表达,显著高于膀胱正常粘膜组织(P<0.01);Pol■在膀胱肿瘤及肾盂癌组织的表达显著高于膀胱正常粘膜组织的表达(P<0.01),且与移行细胞癌的分级相关。结论:Pol■在移行细胞癌组织中的高表达可能与膀胱肿瘤的发生、发展相关,为进一步研究Pol■在膀胱肿瘤中表达的意义打下基础。
Objective: To detect the expression of DNA polymerase (Pol) in bladder tumor cell lines and transitional cell carcinoma and to explore the significance of Pol ¢ ò expression in transitional cell carcinoma. Methods: The bladder cancer cell line BIU87 and T24 cell lines were cultured. The expression of Pol ¢ ¢ s in bladder cancer cell lines was detected by RT-PCR. Tissue samples of human bladder mucosa normal tissues, clinical bladder tumors and renal pelvic carcinoma were collected , The use of RT-PCR method to detect tissue samples Pol ■ expression. Results: Pol ■ was overexpressed in bladder tumor cell lines and was significantly higher than that in normal mucosa (P <0.01). The expression of Pol ■ was significantly higher in bladder tumor and renal pelvic carcinoma than in normal mucosa (P <0.01) ), And with the classification of transitional cell carcinoma. CONCLUSIONS: The overexpression of Pol-2 in transitional cell carcinoma may be related to the occurrence and development of bladder neoplasm, which lays a foundation for further study on the significance of Pol-2 in bladder neoplasm.