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探讨 IGF- 1及其受体在慢性肾功能不全 (CRF)时骨骼肌蛋白质代谢中的作用。用放射免疫分析法检测 CRF大鼠和与其配对喂养的假手术 (SO)对照组大鼠血清和骨骼肌中IGF- 1的含量 ;用液相杂交 /RNase Protection Assay法观察骨骼肌中 IGF- 1和 IGF- 1受体的基因表达 ;用密度扫描法
To investigate the role of IGF-1 and its receptor in the metabolism of skeletal muscle protein in chronic renal failure (CRF). The levels of IGF-1 in serum and skeletal muscle of CRF rats and sham-operated (SO) control rats fed with it were detected by radioimmunoassay. The levels of IGF-1 in skeletal muscle were detected by liquid chromatography / RNase Assay Assay And IGF-1 receptor gene expression; using density scanning