国内外文献未见乙醇导致生精细胞凋亡的研究报道。我们在药酒L（乙醇含量30％）喂饲小鼠的实验中观察到生精细胞凋亡增多现象。实验选用成年雄性昆明种小鼠40只，体重18－22克，随机分为三个实验剂量组和对照组。实验组小鼠分别按药酒L推荐食用量100ml／鼠·日的1倍、2．5倍、5倍（2ml、5ml、10ml／kg体重）剂量灌胃30天。对照组以蒸溜水灌胃。剖杀后取睾丸常规制片HE染色光镜观察。对照组小鼠睾丸检见少量散在初级精母细胞凋亡，凋亡细胞形态为细胞体积变小、核固缩及核外凸小泡形成。实验低剂量组可见少量散在初级精母细胞、次级精母细胞及精子细胞凋亡；中剂量组可见部分生精细胞呈空网状改变，生精细胞凋亡特点基本同低剂量组但凋亡细胞数量稍有增多，另还检见多核凋亡生精细胞。高剂量组曲细精管生精上皮层次稍减少，各级生精细胞凋亡明显增多，可见散在A型糖原细胞凋亡，还可见双核、多核凋亡生精细胞，个别曲细精管腔内有裸核凋亡生精细胞聚集。本实验结果显示：药酒L低剂量组对小鼠生精细胞凋亡影响较小，高剂量组导致生精铀胞凋亡明显增多，有一定剂量效应关系。说明长期过量饮用乙醇有明显诱导生精细胞凋亡的生殖毒性作用，其机理尚待进一步研究。同时可看出，睾丸生精细胞凋亡指标对于评价生 There is no report about ethanol-induced apoptosis of spermatogenic cells in domestic and foreign literatures. We observed an increase in apoptosis of spermatogenic cells in mice fed ethanol Liquor (ethanol content 30%). Forty adult male Kunming mice weighing 18-22 g were randomly divided into three experimental dose groups and control groups. The mice in the experimental group were orally gavaged for 30 days with the doses of 100ml / mouse / day, 2.5 times, 5 times (2ml, 5ml, 10ml / kg body weight) Control group with distilled water gavage. After taking the testes to take routine test HE staining. A small amount of scattered primary spermatocytes were detected in the testis of the control mice. The apoptotic cells were in the shape of smaller cells, nuclear pyknosis and the formation of nucleus-convex vesicles. A small amount of scattered primary spermatocytes, secondary spermatocytes and spermatid were observed in the experimental low-dose group. Part of spermatogenic cells in the medium-dose group showed some changes in the shape of an empty mesh. The characteristics of apoptosis of spermatogenic cells were basically the same as those in the low-dose group A slight increase in the number of dead cells, the other also seized see multi-nuclear apoptotic spermatogenic cells. High-dose group of seminiferous tubules of seminiferous epithelium slightly reduced the level of apoptosis of spermatogenic cells at all levels was significantly increased, showing scattered A-type glycogen cell apoptosis, but also seen dual-nucleated, multi-nuclear apoptotic spermatogenic cells, individual seminiferous tubules Naked nuclear apoptotic spermatogenic cell aggregation. The experimental results show that: low-dose Liquor L had little effect on the apoptosis of spermatogenic cells in mice, high-dose group led to significantly increased apoptosis of germ-derived uranium cells, with a dose-response relationship. Explain the long-term excessive drinking ethanol significantly induce apoptosis of spermatogenic cells reproductive toxicity, the mechanism remains to be further studied. At the same time can be seen, testicular spermatogenic apoptosis indicators for evaluation of Health