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目的:研究自噬相关基因在弥漫大B细胞淋巴瘤中的表达水平及其临床意义。方法:采用实时定量聚合酶链反应法,检测40例弥漫大B细胞淋巴瘤肿瘤组织和20例淋巴结反应性增生组织中自噬相关基因ATG3、ATG5和ATG7的表达水平,分析上述3个基因在弥漫大B细胞淋巴瘤组织及对照组织中的表达差异;同时,在有完整临床资料的30例患者中,结合临床信息,分析不同表达水平与淋巴瘤分期及患者预后等方面的相关性。结果:与反应性增生的淋巴结组织相比,弥漫大B细胞淋巴瘤组织中的ATG5基因表达水平显著降低(ATG3 P=0.9046;ATG5 P=0.0336;ATG7 P=0.4376)。与ATG5基因高表达患者相比,ATG5基因低表达患者中国际预后指数评分为中-高危分期者较多见(P=0.046)。结论:弥漫大B细胞淋巴瘤患者淋巴瘤肿瘤组织存在自噬减少的现象,这可能是影响疾病进展的重要因素及治疗的潜在靶点之一。
Objective: To study the expression of autophagy-related genes in diffuse large B cell lymphoma and its clinical significance. Methods: The expression levels of ATG3, ATG5 and ATG7 in 40 cases of diffuse large B-cell lymphoma and 20 cases of lymph node reactive hyperplasia tissues were detected by real-time quantitative polymerase chain reaction. The expression of ATG3, Diffuse large B-cell lymphoma and control tissues. Meanwhile, in 30 patients with complete clinical data, the correlation between different expression levels and lymphoma staging and patient prognosis was analyzed according to clinical information. Results: ATG5 gene expression was significantly decreased in diffuse large B-cell lymphoma tissues compared with reactive hyperplastic lymph nodes (ATG3 P = 0.9046; ATG5 P = 0.0336; ATG7 P = 0.4376). Compared with patients with high expression of ATG5, the patients with low expression of ATG5 had higher IGR scores than those with high-risk stages (P = 0.046). Conclusions: Autophagy decreases in lymphomas in patients with diffuse large B-cell lymphoma, which may be one of the important factors affecting the progress of the disease and a potential therapeutic target.