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介绍一种改进的高效液相色谱柱的装填方法——稀匀浆装填方法 .该方法使用无毒、低粘度的稀匀浆溶液和较长的合适结构的匀浆罐 .在高于 70 MPa的压力和 5 0 m l/min匀浆输送流量下 ,装填 5 μm ODS填料的反相色谱柱和 Si O2 硅胶的正相色谱柱都获得良好的结果 .柱效可达到 60 0 0 0 /m以上 ;峰对称性好 ,SI=1～ 1 .2 5 ;寿命长 ,经过 5 0 0次进样柱效和峰对称性无明显变化
An improved filling method for high performance liquid chromatography (HPLC) column, a homogenization slurry filling method, uses a nonpoisonous and low viscosity homogenization solution and a longer homogenizing tank of suitable structure. Of the column and a Si02 silica normal phase column with 5 μm ODS packing at a pressure of 50 ml / min and a permeate flow rate of 50 ml / min. ; Peak symmetry, SI = 1 ~ 1 .2 5; long life, after 500 column injection efficiency and peak symmetry no significant change