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已知对表皮自身抗原发生免疫耐受性障碍并形成自身抗体为天疱疮病人的特征。致敏的表皮自身抗原为鳞状上皮细胞的正常表面成分。作者先前已报道在人的唾液中存在有天疱疮表皮自身抗原。本文报道关于从人的唾液中分离天疱疮表皮自身抗原的程序和结果。用天疱疮抗体与鼠舌上皮发生反应以使间接免疫荧光染色反应被阻断的方法测定天疱疮表皮自身抗原。将新鲜的人全唾液与天疱疮病人血清进行孵育从而全部阻断了天疱疮抗体和鼠舌上皮产生间接免疫荧光染色反应。在7名供体(包括1例天疱疮病人)的唾液中测得天疱疮表皮自身抗
It is known that immunological tolerance to epidermal autoantigens and the formation of autoantibodies characterize patients with pemphigus. Sensitized epidermal autoantigen is the normal surface composition of squamous epithelial cells. The authors have previously reported the presence of pemphigus epidermal autoantigens in human saliva. This paper reports on the procedures and results of the isolation of pemphigus epidermal autoantigens from human saliva. Pemphigus epidermal autoantigens were determined by the reaction of pemphigus antibodies with mouse tongue epithelium to block the indirect immunofluorescent staining reaction. Fresh human whole saliva was incubated with pemphigus patient serum to completely block the indirect immunofluorescence staining of pemphigus antibodies and rat tongue epithelium. Pemphigus epidermal autoantibodies were measured in the saliva of 7 donors (including 1 pemphigoid patient)